INPROTEOLYS

Regulation of the Proteasome

The goal of the lab is to examine novel levels of regulation of the proteasome pathway focusing on the mechanisms that control proteasome function and its interaction with protein substrates . Our ongoing studies pursue basic and translational research areas. Our objective is to characterize the regulatory interactions that control the process of protein degradation in the proteasome. We also aim to identify novel ubiquitin pathway substrates by means of an optimized biochemical fractionation of ubiquitin conjugates. The development of these projects will uncover mechanisms of action of the proteasome, which will be integrated in the comprehension of the role of the proteasome in cell physiology and molecular pathology. One of our priorities will be to reveal novel targets in the proteasome pathway for the design of drug therapies. The use of S. cerevisiae, the reference model in the ubiquitin-proteasome system, will allow us to address simultaneously biochemical, genetic, molecular and cell biology approaches in feasible time frames. Our final aim is to create a reference group specialized in proteasome functional characterization, in a moment in which a new era on proteasome research is emerging.

Bernat Crosas

Marta Isasa

Elijah Katz

Veronica Yugo

E-mail

bernat.crosas@ibmb.csic.es

Phone

34 934020191

Fax

34 934034979

Address

C/ Baldiri i Reixac, 15-21 08028 Barcelona

Center

Institute of Molecular Biology of Barcelona

Group Web

Research Lines

1. To study the proteasome mechanism, focusing on the role of the regulatory particle. We are interested in understanding how the proteasome processes substrates delivered from multiple cellular pathways, in terms of selectivity, priority and homeostatic feedback. The regulatory particle of the proteasome is a functional network of interacting subunits and associated factors which define several levels of control of the proteolytic pathway. Our aim is to uncover this network. 2. To define the physiological scope of the ubiquitin-proteasome system. We are interested in characterizing novel substrates of the system. Inversely, we are also interested in developing a method of conditional degradation by ubiquitin independent approaches, in order to create artificial substrates of the proteasome.

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Keywords

ubiquitin-proteasome system